Anderson Lab at The Ohio State University



Clark L. Anderson, M.D. - Professor
anderson.48@osu.edu

Education and Training:

Brown University
Providence, RI
  1955-57 Biology

University of Arizona
Tucson, AZ

AB 1960

1957-60

Chemistry

University of Chicago
School of Medicine


MD 1964

1960-64

Medicine

University of Chicago
Graduate School
 
1964-65

Biochemistry

Professional Experience:

1965-1966 Intern in Internal Medicine, University of Colorado
1966-1969 Major, Medical Corps, U.S. Army, Germany, ‘Vietnam War Doctors’ Draft’
1969-1971 Resident in Internal Medicine, University of Colorado (Dr. Gordon Meiklejohn)
1971-1974 Research Fellow in Immunology, National Jewish Hospital (Drs. Percy Minden and Howard Grey)
1974-1975 Clinical Immunology Fellow, National Jewish Hospital/University of Colorado (Dr. Richard Farr)
1975-1977 Leukemia Society of America Special Fellow, National Jewish Hospital (Dr. Howard Grey)
1977-1982 Assistant Professor, Department of Medicine, University of Rochester
1982-1986 Associate Professor, Departments of Medicine (Immunology Unit) and Microbiology (Assistant Professor), University of Rochester
1986- Professor, Departments of Internal Medicine and of Molecular and Cellular Biochemistry (and of Molecular Genetics, 1987-98), The Ohio State University


Honors:

1975-1977 Leukemia Society of America Special Fellow
1979-1984 Research Career Development Award, NIAID
1987-1991 Medical Biochemistry Study Section, DRG, NIH 
1994 University Distinguished Scholar Award, OSU 
1998-2002 Section Editor, Journal of Immunology 

Selected Publications:

Anderson, C.L., and H.M. Grey. 1974. Receptors for aggregated IgG on mouse lymphocytes: Their presence on thymocytes, thymus derived and bone marrow derived lymphocytes. J. Exp. Med. 139:1175-1188.

Anderson, C.L., R.T. Kubo, and H.M. Grey. 1975. Cytophilic properties of b2-microglobulin. J. Immunol. 114:997-1000.
Anderson, C.L., and H.M. Grey. 1977. Solubilization and partial characterization of cell membrane Fc receptors. J. Immunol. 118:819-825.

Heusser, C.H., C.L. Anderson, and H.M. Grey. 1977. Receptors for IgG: Subclass specificity of receptors on different mouse cell types and the definition of two distinct receptors on a macrophage cell line. J. Exp.Med. 145:1316-1327.

Anderson, C.L., and H.M. Grey. 1978. Physicochemical separation of two distinct Fc receptors on mouse macrophage-like cell lines. J. Immunol. 121:648-652.

Anderson, C.L. 1980. The murine macrophage Fc receptor for IgG2b is lipid dependent. J. Immunol. 125:538-540.

Anderson, C.L., and W.S. Stillman. 1980. The Raji cell assay for immune complexes. Evidence for detection of Raji-directed immunoglobulin G antibody in sera from patients with systemic lupus erythematosus. J. Clin. Invest. 66:353-360.

Anderson, C.L., and G.N. Abraham. 1980. Characterization of the Fc receptor for IgG on a human macrophage cell line, U937. J. Immunol. 125:2735-2741.

Anderson, C.L., and H.L. Spiegelberg. 1981. Macrophage receptors for IgE: Binding of IgE to specific IgE Fc receptors on a human macrophage cell line, U937. J. Immunol. 126:2470-2473.

Anderson, C.L. 1982. Isolation of the receptor for IgG from a human monocyte cell line (U937) and from human peripheral blood monocytes. J. Exp. Med. 156:1794-1806.

Anderson, C.L., J.M. Spence, T.S. Edwards, and J. Nusbacher. 1985. Characterization of a polyvalent antibody directed against the IgG Fc receptor of human mononuclear phago­cytes. J. Immunol. 134:465-470.

Jones, D.H., J. Nusbacher, and C.L. Anderson. 1985. Fc receptor-mediated binding and endocytosis by human mononuclear phagocytes: Monomeric IgG is not endocytosed by U937 cells and monocytes. J. Cell Biol. 100:558-564.

Jones, D.H., R.J. Looney, and C.L. Anderson. 1985. Two distinct classes of IgG Fc receptors on a human monocyte line (U937) defined by differences in binding of murine IgG subclasses at low ionic strength. J. Immunol. 135:3348-3353.

Rosenfeld, S.I., R.J. Looney, J.P. Leddy, D.C. Phipps, G.N. Abraham, and C.L. Anderson. 1985. Human platelet Fc receptor for IgG: Identification as a 40 kD membrane protein shared by monocytes. J. Clin. Invest. 76:2317-2322.

Looney, R.J., G.N. Abraham, and C.L. Anderson. 1986. Human monocytes and U937 cells bear two distinct Fc receptors for IgG. J. Immunol. 136:1641-1647.

Looney, R.J., D.H. Ryan, K. Takahashi, H.B. Fleit, H.J. Cohen, G.N. Abraham, and C.L. Anderson. 1986. Identification of a second class of IgG Fc receptors on human neutro­phils: A 40 kD molecule found also on eosinophils. J. Exp. Med. 163:826-836.

Anderson, C.L., P.M. Guyre, J.C. Whitin, D.H. Ryan, R.J. Looney, and M.W. Fanger. 1986. Monoclonal antibodies to Fc receptors for IgG on human mononuclear phagocytes: Antibody characterization and induction of superoxide production in a monocyte cell line. J. Biol. Chem. 261:12856-12864.

O'Grady, J.H., R.J. Looney, and C.L. Anderson. 1986. The valence for ligand of the human mononuclear phagocyte high affinity IgG Fc receptor is one. J. Immunol. 137:2307-2310.

Anderson, C.L., and R.J. Looney. 1986. Review: Human Leukocyte IgG Fc Receptors. Immunology Today 7:264-266.

Anderson, C.L., and R.J. Looney. 1987. IgG Fc receptors of human leukocytes. Meth. Enz. 150:524-536.

Shen, L., P.M. Guyre, C.L. Anderson, and M.W. Fanger. 1986. Heteroantibody mediated cytotoxicity: Antibody to the high affinity Fc receptor for IgG mediates cytotoxicity by human monocytes which is enhanced by gamma interferon and is not blocked by human IgG. J. Immunol. 137:3378-3383.

Anderson, C.L., D.H. Ryan, R.J. Looney, and P.C. Leary. 1987. Polymorphism of the human monocyte 40 kD receptor for IgG. J. Immunol. 138:2254-2256.

Rosenfeld, S.I., D.H. Ryan, R.J. Looney, C.L. Anderson, G.N. Abraham, and J.P. Leddy. 1987. Human platelet Fc receptors: Quantitative expression correlates with functional responses. J. Immunol. 138:2869-2873.

Looney, R.J., C.L. Anderson, D.H. Ryan and S.I. Rosenfeld. 1987. Structural polymorphism of the human platelet Fc receptor does not correlate with variations in quantitative expression. J. Immunol. 141:2680-2683.

Ceuppens, J.L., M.L. Baroja, F.V. Vaeck and C.L. Anderson. 1988. A defect in the membrane expression of high affinity 72 kD Fc receptors on phagocytic cells in four healthy subjects. J. Clin. Invest. 82:571-578.

Peltz, G., K. Frederick, C.L. Anderson and B.M. Peterlin. 1988. Characterization of the human monocyte high affinity Fc receptor. Mol. Immunol. 25:243-250.

Anderson, G.P. and C.L. Anderson. 1990. Signal transduction by the platelet Fc receptor. Blood. 76:1665-1172.

Ravetch, J.V. and C.L. Anderson. 1990. The FcgR family: Proteins, transcripts and genes. In Fc Receptors and the Action of Antibodies. Ed. H. Metzger, American Society of Microbiology. pp 211-238.

Anderson, C.L., L. Shen, D.M. Eicher, M.D. Wewers, and J.K. Gill. 1990. Phagocytosis mediated by three distinct Fcg receptor classes on human leukocytes. J. Exp. Med. 171:1333-1346.

Sedmak, D.D., D.H. Davis, U. Singh, and C.L. Anderson. 1991. Distribution of IgG Fc Receptors in the Human Placenta and on Endothelial Cells: An Immunohistochemical Study. Am. J. Path. 138:175-181.

Anderson, C.L., R.J. Looney, D.J. Culp, D.H. Ryan, H.B. Fleit, M.J. Utell, M.W. Frampton, P.D. Manganiello, and P.M. Guyre. 1990. Alveolar and peritoneal macrophages bear three distinct classes of Fc receptors for IgG. J. Immunol. 145:196-201.

Anderson, G.P., J.G.J. van de Winkel, and C.L. Anderson. 1991. Anti-CD41 monoclonal antibody-induced platelet activation requires Fc receptor-dependent cell-cell interaction. Br. J. Hematology. 79:75-83.

Van de Winkel, J.G.J., L.K. Ernst, C.L. Anderson, and I.-M. Chiu. 1991. Gene organization of the human high-affinity receptor for IgG, FcgRI (CD64). Characterization and evidence for a second gene. J. Biol. Chem. 266:13449-13455.

Van de Winkel, J.G.J. and C.L. Anderson. 1991. Biology of human IgG Fc receptors. J. Leuko. Biol. 49:511-524. (Outstanding Review Award, 1996)

Ernst, L.K., J.G.J. Van de Winkel, I.-M. Chiu and C.L. Anderson. 1992. Three genes for the human high-affinity receptor for IgG (FcgRI) encode four distinct transcripts. J. Biol. Chem. 267:15692-15700.

Lairmore, M.D., Cuthbert, P.S., Utley, L.L., Morgan, C.J., Dezzutti, C.S., Anderson, C.L., and D.D. Sedmak. 1993. Cellular Localization of CD4 in the Human Placenta: Implications for Maternal-to-Fetal Transmission of Human Immunodeficiency Virus. J. Immunol. 151:1673-1681.

Ernst, L.K., A.-M. Duchemin, and C.L. Anderson. 1993. Association of the high affinity receptor for IgG (FcgRI) with the gamma subunit of the IgE receptor. Proc. Natl. Acad. Sci. USA. 90:6023-6027.

Osborne, J.M., G.W. Chacko, J.T. Brandt, and C.L. Anderson. 1994. Ethnic variation in frequency of FcgRIIa high responder/low responder alleles as determined with allele specific oligonucleotide probes. J. Immunol. Methods. 173:207-217.

Duchemin, A.-M., L.K.Ernst, and C.L. Anderson. 1994. Clustering of the high affinity Fc receptor for IgG (FcgRI) results in phosphorylation of its association FcRg-chain. J. Biol. Chem. 269:12111-12117.

Chacko, G.C., A.-M. Duchemin, K.M.Coggeshall, J.M.Osborne, J.T.Brandt, and C.L. Anderson. 1994. Clustering of the platelet FcgRII induces noncovalent association with the tyrosine kinase p72syk. J. Biol. Chem. 269:32435-32440.

Leach, J.L., D.D. Sedmak, M.D. Lairmore, and C.L. Anderson. 1996. Isolation from human placenta of the IgG transporter, FcRn and localization to the syncytiotrophoblast: Implications for maternal-fetal antibody transport. J. Immunol. 157:3317-3322.

Brandt, J.T., C.E. Isenhart, J.M. Osborne, A. Ahmed, and C.L. Anderson. 1995. On the role of platelet FcgRIIA phenotype in heparin-induced thrombocytopenia. Thrombosis and Haemostasis. 74:1564-1572.

Maresco, D.L., E. Chang, K.S. Theil, U. Franke, and C.L. Anderson. 1995. The three genes of the human FcgRI gene family flank the centromere of chromosome 1 at 1p12 and 1q21. Cell Genetics and Cytogenetics. 73:157-163.

Miller, K.L., A.-M. Duchemin, and C.L. Anderson. 1996. A novel role for the Fc receptor g subunit: Enhancement of FcgR ligand affinity. J. Exp. Med. 183:2227-2234.

Chacko, G.W., J.T.Brandt, K.M. Coggeshall, and C.L. Anderson. 1996. Phosphatidylinositol 3-kinase and p72syk noncovalently associate with the low affinity Fcg on human platelets through an ITAM: Reconstitution with synthetic phosphopeptides. J. Biol. Chem. 271:10775-10781.

Junghans, R.P. and C.L. Anderson. 1996. The protection receptor for IgG catabolism is the b2-microglobulin-containing neonatal intestinal transport receptor. Proc. Natl. Acad. Sci. 93:5512-5516.

Duchemin, A.-M. and C.L. Anderson. 1997. Association of non-receptor protein tyrosine kinases with the FcgRI g-chain complex in monocytic cells. J. Immunol. 158:865-871

Brandt, J.T., C.J. Julius, J.M. Osborne, and C.L. Anderson. 1996. The mechanism of platelet aggregation induced by HLA-related antibodies. Thrombosis and Haemostasis. 76:774-779.

Marsh, C.B., C.L. Anderson, M.P. Lowe, and M.D. Wewers. 1996. Monocyte IL-8 release is induced by two independent Fc gamma R-mediated pathways. J. Immunol. 157:2632-2637.

Sedmak, D.D., B.M. Rahill, J.M. Osborne, J.L. Leach, M.D. Lairmore, R.P. Junghans, and C.L. Anderson. 1996. Human endothelial cells express FcRp, the IgG protection receptor. Submitted.

Liu, Z., D.C. Roopenian, X. Zhou, L.A. Diaz, D.D. Sedmak, and C.L. Anderson. 1997. b2-microglobuolin-deficient mice are resistant to bullous pemphigoid. J. Exp. Med. 186:777-784.

Lowry, M.B., A.-M. Duchemin, J.M. Robinson, and C.L. Anderson. 1998. Functional separation of pseudopod extension and particle internalization during Fcg receptor-mediated phagocytosis. J. Exp. Med. 187:161-176.

Ernst, L.K., A.-M. Duchemin, K.L. Miller, and C.L. Anderson. 1998. Molecular Characterization of Six Variant Fcg Receptor Class I (CD64) Transcripts. Mol. Immunol. 35:943-954

Maresco, D.L., L.E. Blue, L.L. Culley, R.P.Kimberly, C.L. Anderson, and K.S. Theil. 1998. Localization of FCGR1 encoding FcgRI in primates: Molecular evidence for 2 pericentric inversions during the evolution of human chromosome 1. Cell Genetics and Cytogenetics. 82:71-74.

Lowry, M.B, A.-M. Duchemin, K.M. Coggeshall, J.M. Robinson, and C.L. Anderson. 1998. Chimeric receptors composed of PI3-kinase domains and Fcg receptor ligand-binding domains mediate phagocytosis in COS fibroblasts. J. Biol. Chem. 273:24513-24520.

Maresco, D.L., J.M. Osborne, D. Cooney, F.M. Coggeshall, and C.L. Anderson. 1999. The SH2-containing 5’-inositol phosphatase (SHIP) is tyrosine phosphorylated after Fcg receptor clustering in monocytes. J. Immunol. 162:6458-6465

Tridandapani, S., M. Pra, J.R. LaDine, S. Garber, C.L. Anderson, and K.M. Coggeshall, 1999. Protein interactions of src homology 2 (SH2) domain-containing inositol phosphatase (SHIP): Association with Shc displaces SHIP from FcgRIIb in B cells. J. Immunol. 162:1408-1414

Miller, K.L., A.-M. Duchemin, L.K. Ernst, and C.L. Anderson. 2000. Characterization of the two-extracellular domain form of Fcg receptor class I: A product of the B gene. Submitted.

Miller, K.L., S.A. Garber, A.-M. Duchemin, and C.L. Anderson. 2000. Homodimerization of the ligand-binding a-chains of the high affinity Fc receptor for IgG (FcgRI). Submitted.

Tridandapani,S, T.W. Lyden, J.L. Smith, K.M. Coggeshall, and C.L. Anderson. 2000. The adapter protein LAT enhances FcgR-mediated signal transduction in myeloid cells. J. Biol. Chem. 275:20480-20487.

Lyden, T.W., J.M. Robinson, S. Tridandapani, J.-P.Teillaud, S.A.Garber, J.M. Osborne, J. Frey, P. Budde, C.L. Anderson. 2001. The Fc receptor for IgG expressed in the villus endothelium of human placenta is FcgRIIb2. J. Immunol. 166:3882-3889.

Tridandapani,S., K. Siefker, J.-L. Teillaud, J.E. Carter, M.D. Wewers, and C.L. Anderson. 2002. Regulated expression and inhibitory function of FcgRIIb in human monocytic cells. J. Biol. Chem. 277: 5082-5089.

Lyden, T.W., C.L. Anderson, and J.M. Robinson. 2002. The endothelium but not the syncytiotrophoblast of human placenta expresses caveolae. Placenta. 23:640-652.

Tridandapani, S., Y. Wang, C.B. Marsh, and C.L. Anderson. 2002. SH2 domain-containing inositol phosphatase (SHIP) regulates NFkB-dependent gene transcription by phagocytic FcgR. J. Immunol. 169:4370-4378.

Takizawa,T., C.L. Anderson, and J.M. Robinson. 2003. A new method to enhance contrast of ultrathin cryosections for immunoelectron microscopy. Journal of Histochemistry and Cytochemistry 51: 31-39.

Roopenian, D.C., G.J. Christianson, T.J. Sproule, A.C. Brown, S. Akilesh, N. Jung, S. Petkova, E.Y. Choi, L. Avanessyan, D.J. Shaffer, and C.L. Anderson. 2003. The MHC Class I-like IgG receptor (FcRn) controls perinatal IgG transport, IgG homeostasis, and the fate of IgG-Fc-coupled drugs. J. Immunol. 170: 3528-3533.

Chaudhury, C., S. Mehnaz, J.M. Robinson, W.L. Hayton, D.K. Pearl, D.C. Roopenian, and C.L. Anderson. 2003. The MHC-related Fc receptor for IgG binds albumin and prolongs its lifespan. J. Exp. Med. 197:315-322

Pengal, R.A., L. P. Ganesan, H. Fang, C.B. Marsh, C.L. Anderson, and S. Tridandapani. 2003. SHIP-2 inositol phosphatase is inducibly expressed in human monocytes and serves to regulate Fcg receptor-mediated signaling. J. Biol.Chem. 278(25):22657-22663.

Takizawa, T, Anderson, C.L., and J.M. Robinson.  2005.  A novel FcγR-defined, IgG-containing organelle in placental endothelium. J. Immunol., 175: 2331-2339.

Chaudhury C, Brooks CL, Carter DC, Robinson JM, and CL Anderson. 2005. Albumin binding by the MHC-related Fc receptor (FcRn): Albumin and IgG are salvaged independently by similar mechanisms. Biochemistry. 45:4983-4990

Wani, MA, Haynes, LD, Kim, J, Bronson, CL, Chaudhury, C, Mohanty, S, Waldmann, TA, Robinson, JM, CL Anderson. 2006. Familial hypercatabolic hypoproteinemia caused by FcRn deficiency due to mutant ß2-microglobulin gene.  Proc. Natl. Acad. Sci. 103:5084-5089.

Bronson, CL, J Kim, C Chaudhury, WL Hayton, TM Oberyszyn, DC Roopenian, JM Robinson, and CL Anderson.  2006. The MHC-related receptor for IgG, FcRn, is a mediator of the transendothelial flux of albumin and IgG.  Submitted.

Kim, J, CL Bronson, WL Hayton, MD Radmacher, DC Roopenian, JM Robinson, and CL Anderson, 2006.  Albumin turnover: FcRn-mediated recycling saves as much albumin from degradation the liver produces.  Am.J.Physiol. 290:G352-G360. 

Kim, J, WL Hayton, JM Robinson, CL Anderson. 2007. Kinetics of FcRn-mediated recycling of IgG and albumin in human: Pathophysiology and therapeutic implications using a simplified mechanism-based model.  Clin. Immunol. 122:146-155. 

Anderson CL, C Chaudhury, J Kim, CL Bronson MAWani, S Mohanty. 2006. A perspective: FcRn transports albumin: relevance to immunology and medicine.  Trends Immunol. 27:343-8.

Chaudhury C, Kim J. Mehnaz S, Wani MA, Oberyszyn TM, Hayton WL, Robinson JM, Anderson CL.  2007. Accelerated transferrin degradation in HFE-deficient mice is associated with increased transferrin saturation. J.Nutr. 136:2993-2998. 

Kim J, Bronson CL, Wani WA, Oberyszyn TM, Mohanty S, Chaudhury C, Hayton WL, Robinson JM, and CL Anderson.  2008. β2-Microglobulin deficient mice catabolize IgG more rapidly than FcRn-α-chain deficient mice.  Exp Biol Med.  233:603-609.

Kim J, Mohanty S, Ganesan LP, Hua K, Jarjoura D, Hayton WL, Robinson JM and CL Anderson.  2009. FcRn in the yolk sac endoderm of mouse is required for IgG transport to fetus.  J. Immunol. 182:2583-2589.  PMC2676880

Mohanty S, Kim J, Ganesan LP, Phillips GS, Hua K, Jarjoura D, Hayton WL, Robinson JM, and CL Anderson.  2010.  IgG is transported across the mouse yolk sac independently of FcγRIIb.  J. Reprod. Immunol. 84:133-144.

Ganesan LP, Mohanty  S, Kim J, Clark KR, Robinson JM, and CL Anderson.  2011.  Rapid and efficient clearance of blood-borne virus by liver endothelium.  PLoS Pathogens.  7(9): e1002281. doi:10.1371/journal.ppat.1002281

Ganesan LP, Kim J, Wu Y, Mohanty S, Phillips GS, Birmingham DJ, Robinson JM, and CL Anderson. 2012. FcγRIIb on liver sinusoidal endothelium clears small immune complexes. J. Immunol. 189(10):4981-8. doi: 10.4049/jimmunol.1202017. Epub 2012 Oct 10. PMID: 23053513

Mohanty S, Kim J, Ganesan LP, Phillips GS, Robinson JM, and CL Anderson, 2013. Abundant intracellular IgG in enterocytes and endoderm lacking FcRn. PLoS One. 2013 Jul 29;8(7):e70863. doi: 10.1371/journal.pone.0070863. Print 2013. PMID: 23923029

Anderson, CL. 2013. Alternative Explanation, eLetter-to-Editor about Zhi et al paper, Cooperative integrin/ITAM signaling in platelets enhances thrombus formation in vitro and in vivo. Blood. 121:1858-1867. PMID 23264598

Ganesan LP, Mohanty S, Kim J, Clark KR, Robinson JM, and CL Anderson. 2011. Rapid and efficient clearance of blood-borne virus by liver endothelium. PLoS Pathogens. 7(9): e1002281. doi:10.1371/ journal.ppat.1002281. PMID 21980295

Anderson CL & J Kim. 2013. Preview: Surmounting an Impasse of FcRn Structure. Structure. 2013 Nov 5;21(11):1907-8.doi: 10.1016/j.str.2013.10.009. PMID 24210225

 


Room 012k Heart and Lung Institute (HLRI)
473 West Twelfth Avenue
Columbus, Ohio 43210-1228
Office: 614-247-7650
Lab: 614-247-7654

Fax: 614-247-7669
anderson.48@osu.edu